Clostridium scindens strain闪烁拟杆菌菌株ATCC 35704 BioVector NTCC质粒载体菌种细胞基因保藏中心

Clostridium scindens strain闪烁拟杆菌菌株ATCC 35704

Clostridium scindens strain VPI 13733 is a whole-genome sequenced type strain that was isolated from feces. This bacterial culture produces 20 alpha-hydroxysteroid dehydrogenase, 7 beta-dehydrogenase, 7 alpha-dehydroxylase, bile acid, and the steroid desmolase.
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Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI 13733
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Geographical isolation
United States; New York
Product format
Freeze-dried
Storage conditions
2°C to 8°C

General
Specific applications
Produces 20 alpha-hydroxysteroid dehydrogenase, 7 beta-dehydrogenase, 7 alpha-dehydroxylase, bile acid, and the steroid desmolase
Preceptrol
No
Characteristics
Comments
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project

Handling information
Medium
NTCC® Medium 2107: Modified Reinforced Clostridial
NTCC® Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically rehydrate the entire pellet with approximately 0.5 mL of #2107 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2107 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a Brucella Blood plate and/or #260 agar slant.
Incubate in an anaerobic atmosphere at 37°C for 24 to 48 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained
Handling notes
This strain has been sequenced as a reference genome for the NIH Human Microbiome Project.
Anaerobe Systems Brucella Blood Agar plates (AS-111 or AS-141) are recommended for analyzing colony morphology and purity.

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