pLEM415-ldhL-mRFP1 plasmid vector乳酸菌红色荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLEM415-ldhL-mRFP1 plasmid vector乳酸菌红色荧光表达质粒载体

Expression of RFP in Lactobacillus

BACKBONE
Vector backbonepLEM415
(Search Vector Database)
Backbone manufacturerMichel Fons
Backbone size w/o insert (bp)6332
Total vector size (bp)7357
Modifications to backbonethe ldhl promoter was inserted 5’ of the FLAG-mRFP1
Vector typeBacterial Expression
Selectable markersErythromycin
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH5alpha
Growth instructionsLB medium
Copy numberHigh Copy
GENE/INSERT
Gene/Insert namemonomeric red fluorescent protein
Alt namemRFP1
SpeciesDiscosoma coral
Insert Size (bp)1025
GenBank IDAF506025
Promoterldhl
Tag / Fusion Protein
FLAG (N terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteApaI (not destroyed)
3′ cloning siteClaI (not destroyed)
5′ sequencing primerGCAAGGCGATTAAGTTGGGTAAC
3′ sequencing primerACGGTAAAACCATCGACCAATATCT

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net