HEK293 cell line细胞株ECACC 85120602 BioVector NTCC质粒载体菌种细胞基因保藏中心

HEK293 cell line细胞株ECACC 85120602

Cell Line Name: 293
Collection: ECACC General Collection
Catalogue No.: 85120602
BioVector NTCC Inc.

Other Collection No.: CRL 1573
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: 293 (ECACC 85120602)
Keywords: Human Embryo Kidney
Cell Line Description: Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.
PLEASE NOTE:  293 cells detach at room temperature; may take up to seven days to reattach.

Species: Human
Tissue of Origin: Kidney, embryo
CellType: Epithelial
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Karyotype: 2n 46, hypotriploid, modal no. 64
GMO Status: Genetically Modified Organism Class 1 (GMO1)
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 5 x10,000/cm². Cells may take up to 7 days to attach after resuscitation and subculture. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and reseed to allow re-attachment of cells. These cells should be frozen in 5% DMSO : 95% FCS
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FCS

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