P47818 · CCC1 YEAST mutant strain酵母突变株 BioVector NTCC质粒载体菌种细胞基因保藏中心

P47818 · CCC1 YEAST mutant strain酵母突变株

Introduction
P47818 · CCC1_YEAST
Protein: Protein CCC1
Gene:CCC1
Status:UniProtKB reviewed (Swiss-Prot)
Organism:Saccharomyces cerevisiae (strain S288c) (Baker's yeast)
Amino acids: 322
Protein existence: Evidence at protein level

Disruption phenotype
Deletion causes an increase in metal sensitivity at 15 mM manganese, but does not affect invertase glycosylation. Deletion shows increased sensitivity to external iron, which is suppressed by MRS3 or MRS4 overexpression requiring oxygen but not respiration, and exacerbated by ectopic expression of the iron transporter FET4. Deletion also results in decreased vacuolar iron content and decreased iron stores, which affect cytosolic iron levels and cell growth. Deletion together with MRS3 and MRS4 restores cellular and mitochondrial iron homeostasis to near normal level, corrects the MRS3 and MRS4 double deletion phenotype (which shows increased resistance to cobalt but decreased resistance to copper and cadmium), and has near normal levels of aconitase activity. When overexpressed, maintains respiratory function in a YFH1 deletion mutant regardless of extracellular iron concentration, activates the iron-dependent transcription factor AFT1 resulting in an increase in iron uptake, cytosolic iron accumulation and a change in copper metabolism. Overexpression prevents excessive mitochondrial iron accumulation by limiting mitochondrial iron uptake and results in increased expression of the high affinity iron transport system composed of FET3 and FTR1. Overexpression also suppresses the rapamycin-resistant phenotype of PMR1 deletion mutant.

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