SEM cell line细胞株ACC 546 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98965
- 货 号：DSM ACC 546
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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SEM cell line细胞株ACC 546
Cell line: SEM NTCC® no.: ACC 546 Species: human (Homo sapiens) Cell type: B cell precursor leukemia Origin: established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) Reference(s): 14577, 14493, 14887, 40437, 18187 Biosafety level: 1 Permissions and restrictions: A NTCC® Cell Culture Data: Morphology: round to polygonal single rather small cells in suspension; image; image; image Medium: 90% Iscove's MDM + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 30 hours Harvest: cell harvest of ca. 3-4 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO NTCC® Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology: CD3-, CD4-, CD11b-, CD13+, CD14-, CD15+, CD19+, CD33+, CD34-, cyCD68-, HLA-DR+, sm/cyIgG-, sm/cyIgM-, sm/cykappa-, sm/cylamda-; image Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. Cytogenetics: human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype Molec. Genetics: expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Cell line: SEM NTCC® no.: ACC 546 Species: human (Homo sapiens) Cell type: B cell precursor leukemia Origin: established from the peripheral blood of a 5-year-old girl at relapse of acute lymphoblastic leukemia (ALL) in 1990; reported to carry the t(4;11) KMT2A-AFF1 (MLL-AFF1; MLL-AF4). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) Reference(s): 14577, 14493, 14887, 40437, 18187 Biosafety level: 1 Permissions and restrictions: A NTCC® Cell Culture Data: Morphology: round to polygonal single rather small cells in suspension; image; image; image Medium: 90% Iscove's MDM + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:6 every 2-3 days; maximum density at ca. 3-4 x 106 cells/ml Incubation: at 37 °C with 5% CO2 Doubling time: ca. 30 hours Harvest: cell harvest of ca. 3-4 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO NTCC® Scientific Data: Mycoplasma: negative in microbiological culture, PCR assays Immunology: CD3-, CD4-, CD11b-, CD13+, CD14-, CD15+, CD19+, CD33+, CD34-, cyCD68-, HLA-DR+, sm/cyIgG-, sm/cyIgM-, sm/cykappa-, sm/cylamda-; image Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. Cytogenetics: human hypodiploid karyotype with 1.5% polyploidy - 45(40-46)<2n>XX, -13, t(4;11)(q21;q23), del(7)(p14) - carries t(4;11) with breakpoints at AFF1 (AF4) and KMT2A (MLL) - matches published karyotype Molec. Genetics: expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) confirmed by RT-PCR Viruses: PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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