TMO311 strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

TMO311 strain菌株

Strain No : TMO310 Resource Information Organism Prokaryotes (B .subtilis) strain or clone Strain Category KAO/NAIST strains Resource name TMO310 Availability Available Features genotype : trpC2,aprE::pAPNC213-chpA(spec) Taxonomy : Bacillus subtilis Reference : Morimoto T, Ara K, Ozaki K, Ogasawara N. (2011) A simple method for introducing marker-free deletions in the Bacillus subtilis genome. Methods Mol Biol 765 345-58 Morimoto T, Ara K, Ozaki K, Ogasawara N. (2009) A new simple method to introduce marker-free deletions in the Bacillus subtilis genome. Genes Genet Syst 84(4) 315-8 TMO311. trpC2 aprE::(kan lacI Pspac-mazF). [20] ; YNB001. trpC2 comK::spc. This study ; YNB022. trpC2 pLS20cat (kan lacI Pspac-mazF). This study ; YNB026. trpC2  Strain TMO311 (aprE::kan) was transformed with the recombinant PCR fragment to select colonies resistant both to erythromycin and kanamycin.

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