RH-18 cell line人肺泡横纹肌肉瘤细胞株RH18 ACC 491 BioVector NTCC质粒载体菌种细胞基因保藏中心

RH-18 cell line人肺泡横纹肌肉瘤细胞株RH18 ACC 491

Cell line:
RH-18
NTCC® no.:
NTCC®-ACC491

Species:
human (Homo sapiens)
Cell type:
rhabdomyosarcoma

Origin:
cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation

NTCC® Cell Culture Data:
Morphology:
large transparent epitheloid cells, partially with long extensions, growing as monolayers;


Medium:
80% RPMI 1640 + 20% h.i. FBS

Subculture:
seed out at ca. 0.5-1 x 10^6 cells/175cm2; cells grow rather slowly after thawing; later, split culture 1:3 every 3-6 days (before reaching confluency) using trypsin/EDTA; confluency of the culture might result in reduced growth rate after passaging.
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
maximum cell harvest of ca. 4 x 10^6 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

NTCC® Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays

Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@NTCC®.de.

Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

Cytogenetics:
human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -


Cell line name Rh18
Synonyms RH18; RH-18; Rh-18; Rh 18; SJRH-18; SJRH18
Accession NTCC®_1659
Secondary accession NTCC®_5918
Resource Identification Initiative To cite this cell line use: Rh18 (NTCC®_1659)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.
Characteristics: Established from a immuno-deficient mice xenograft.
Doubling time: 83 hours (PubMed=20922763); ~60-70 hours (DSMZ=ACC-491); 65 hours (COG).
Microsatellite instability: Instable (MSI-low) (Sanger).
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Sequence variations
Gene fusion; HGNC; 3819; FOXO1 + HGNC; 8617; PAX3; Name(s)=PAX3-FOXO1, PAX3-FKHR (PubMed=7536457; PubMed=8275086; PubMed=23578105).
Mutation; HGNC; 11998; TP53; None_reported; -; Zygosity=- (PubMed=9802058).
Genome ancestry Source: PubMed=30894373

Origin % genome
African 74.53
Native American 0.16
East Asian, North 4.19
East Asian, South 0
South Asian 0
European, North 5.05
European, South 16.06
Disease Alveolar rhabdomyosarcoma (NCIt: C3749)
Alveolar rhabdomyosarcoma (ORDO: Orphanet_99756)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_DG02 (Rh18c)
Sex of cell Female
Age at sampling 2Y
Category Cancer cell line


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