Yarrowia lipolytica (strain CLIB 122 / E 150) strain解脂耶民酵母E150 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号:Yarrowia lipolytica (strain CLIB 122 / E 150 E150
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Yarrowia lipolytica (strain CLIB 122 / E 150) strain解脂耶民酵母E150
Strain Designations E150Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) YarrowAlternate State Candida lipolytica (Harrison) Diddens et LodderApplication transformation hostMedium YPD MediumBroth MediumYPD Broth(BD 242820)……………………....50 gDI Water………………………………………..1000 mlOr for solid medium:Agar MediumYPD Agar(BD 242720)………………………..65 gDI Water………………………………………...1000 mlDispense into appropriate vessel and autoclave at 121ºC.*YPD is commercially available from BD. To make medium from scratch, followformulation below:Yeast Extract……………………………………10.0 gPeptone…………………………………………20.0 gDextrose………………………………………...20.0 gAgar (if needed)………………………………..20.0 gDI Water………………………………………...1000 mlAdjust for final pH of 5.6. Autoclave at 121oC.Growth Conditions Temperature: 24°C to 26°CAtmosphere: Typical aerobicGenotype MatB, his1, leu2-270, ura3-302, xpr2-322Comments E150 is a haploid derivative of a cross of CBS6124-2 isolated from an American corn processing plant and CLIB89. Morphology On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Strain Designations E150Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) YarrowAlternate State Candida lipolytica (Harrison) Diddens et LodderApplication transformation hostMedium YPD MediumBroth MediumYPD Broth(BD 242820)……………………....50 gDI Water………………………………………..1000 mlOr for solid medium:Agar MediumYPD Agar(BD 242720)………………………..65 gDI Water………………………………………...1000 mlDispense into appropriate vessel and autoclave at 121ºC.*YPD is commercially available from BD. To make medium from scratch, followformulation below:Yeast Extract……………………………………10.0 gPeptone…………………………………………20.0 gDextrose………………………………………...20.0 gAgar (if needed)………………………………..20.0 gDI Water………………………………………...1000 mlAdjust for final pH of 5.6. Autoclave at 121oC.Growth Conditions Temperature: 24°C to 26°CAtmosphere: Typical aerobicGenotype MatB, his1, leu2-270, ura3-302, xpr2-322Comments E150 is a haploid derivative of a cross of CBS6124-2 isolated from an American corn processing plant and CLIB89. Morphology On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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