首页 » Yarrowia lipolytica (strain CLIB 122 / E 150) strain解脂耶民酵母E150 BioVector NTCC质粒载体菌种细胞基因保藏中心

Yarrowia lipolytica (strain CLIB 122 / E 150) strain解脂耶民酵母E150 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥98950
  • 货  号:Yarrowia lipolytica (strain CLIB 122 / E 150 E150
  • 产  地:北京
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Yarrowia lipolytica (strain CLIB 122 / E 150) strain解脂耶民酵母E150

Strain Designations E150
Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) Yarrow
Alternate State Candida lipolytica (Harrison) Diddens et Lodder
Application transformation host
Medium YPD Medium
Broth Medium
YPD Broth(BD 242820)……………………....50 g
DI Water………………………………………..1000 ml
Or for solid medium:
Agar Medium
YPD Agar(BD 242720)………………………..65 g
DI Water………………………………………...1000 ml
Dispense into appropriate vessel and autoclave at 121ºC.
*YPD is commercially available from BD. To make medium from scratch, follow
formulation below:
Yeast Extract……………………………………10.0 g
Peptone…………………………………………20.0 g
Dextrose………………………………………...20.0 g
Agar (if needed)………………………………..20.0 g
DI Water………………………………………...1000 ml
Adjust for final pH of 5.6. Autoclave at 121oC.
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Genotype MatB, his1, leu2-270, ura3-302, xpr2-322
Comments E150 is a haploid derivative of a cross of CBS6124-2 isolated from an American corn processing plant and CLIB89.
Morphology On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.

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