pYeDP60u2 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98950
- 货 号：pYeDP60u2
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pYeDP60u2 plasmid vector质粒载体
The WAT11 yeast strain was transformed with pYeDP60u2 containing the different P450 sequences The generation of the CYP76B1 construct is described in Didierjean et al. (2002). All other constructs are described in Höfer et al. (2013). The yeast (Saccharomyces cerevisiae) and plant expression constructs were generated by PCR amplification from complementary DNA prepared from tissues in which each gene was found to be the most highly expressed. The PCR fragments of CYP76C1, CYP76C2, CYP76C5, CYP76C7, and CYP76B1 were integrated into the yeast expression vector pYeDP60. The constructs for CYP76C3, CYP76C4, CYP76C6, and CYP76B6 were prepared using the Uracil-Specific Excision Reagent (New England Biolabs) cloning technique according to Nour-Eldin et al. (2006) and the PCR fragments were integrated into the yeast expression plasmid pYeDP60u2. For plant expression constructs, CYP76C1 was cloned similarly and integrated in the plant expression vector pCAMBIA2300u. Complementary DNA from CYP76C2 and CYP76C4 was amplified by PCR using specific primers tailed for Gateway cloning technology (Invitrogen) and successively cloned in pDONR 201 and the plant expression vector pB7WG2 (Karimi et al., 2002). Constructs were confirmed by sequencing at each step.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
The WAT11 yeast strain was transformed with pYeDP60u2 containing the different P450 sequences The generation of the CYP76B1 construct is described in Didierjean et al. (2002). All other constructs are described in Höfer et al. (2013). The yeast (Saccharomyces cerevisiae) and plant expression constructs were generated by PCR amplification from complementary DNA prepared from tissues in which each gene was found to be the most highly expressed. The PCR fragments of CYP76C1, CYP76C2, CYP76C5, CYP76C7, and CYP76B1 were integrated into the yeast expression vector pYeDP60. The constructs for CYP76C3, CYP76C4, CYP76C6, and CYP76B6 were prepared using the Uracil-Specific Excision Reagent (New England Biolabs) cloning technique according to Nour-Eldin et al. (2006) and the PCR fragments were integrated into the yeast expression plasmid pYeDP60u2. For plant expression constructs, CYP76C1 was cloned similarly and integrated in the plant expression vector pCAMBIA2300u. Complementary DNA from CYP76C2 and CYP76C4 was amplified by PCR using specific primers tailed for Gateway cloning technology (Invitrogen) and successively cloned in pDONR 201 and the plant expression vector pB7WG2 (Karimi et al., 2002). Constructs were confirmed by sequencing at each step.
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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