FCWF cell line细胞株Fcwf-4 ATCC CRL-2787 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98950
- 货 号：FCWF
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FCWF cell line细胞株Fcwf-4 ATCC CRL-2787
Fcwf-4 [Fcwf]NTCC®-CRL-2787Product categoryAnimal cellsOrganismFelis catus, catCell typemacrophageMorphologyspindle to stellateTissueFetus; WholeDiseasePeritonitisApplications3D cell cultureImmunologyGeneralSpecific applicationsIt is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV).CharacteristicsGrowth propertiesAdherentDerivationThis continuous feline cell line was developed in 1979. AgefetusVirus susceptibilityFeline infectious peritonitis virusCommentsThe cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors).Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.It is recommended that the cryoprotective agent be removed immediately. Centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cell pellet in an appropriate amount of fresh growth medium.Transfer the vial contents to an appropriate size vessel. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.An inoculum of 1 x 104 to 1 x 105 viable cells/cm2 is recommended. Do not exceed 1 x 106 cells/cm2.Incubate cultures at 37°C.Interval: Maintain cultures at a cell concentration between 2 x 104 and 1 x 105 cells/cm2.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: Two to three times weeklyReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 31 hrs
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
Fcwf-4 [Fcwf]NTCC®-CRL-2787Product categoryAnimal cellsOrganismFelis catus, catCell typemacrophageMorphologyspindle to stellateTissueFetus; WholeDiseasePeritonitisApplications3D cell cultureImmunologyGeneralSpecific applicationsIt is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV).CharacteristicsGrowth propertiesAdherentDerivationThis continuous feline cell line was developed in 1979. AgefetusVirus susceptibilityFeline infectious peritonitis virusCommentsThe cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors).Handling informationUnpacking and storage instructionsCheck all containers for leakage or breakage.Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below _x001f_-130°C, preferably in liquid nitrogen vapor, until ready for use.Complete mediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature37°CAtmosphere95% Air, 5% CO2Handling procedureTo insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at –70°C. Storage at –70°C will result in loss of viability.Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.It is recommended that the cryoprotective agent be removed immediately. Centrifuge the cell suspension at approximately 125 x g for 5 to 10 minutes. Discard the supernatant and resuspend the cell pellet in an appropriate amount of fresh growth medium.Transfer the vial contents to an appropriate size vessel. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6). Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.Subculturing procedureVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.An inoculum of 1 x 104 to 1 x 105 viable cells/cm2 is recommended. Do not exceed 1 x 106 cells/cm2.Incubate cultures at 37°C.Interval: Maintain cultures at a cell concentration between 2 x 104 and 1 x 105 cells/cm2.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: Two to three times weeklyReagents for cryopreservationComplete growth medium supplemented with 5% (v/v) DMSO (ATCC 4-X)Quality control specificationsMycoplasma contaminationNot detectedPopulation doubling timeApproximately 31 hrs
Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net
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