首页 » pIIIA/IRE-MS2 and pAD-IRP plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIIIA/IRE-MS2 and pAD-IRP plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49850
  • 货  号:pIIIA/IRE-MS2 and pAD-IRP
  • 产  地:北京
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pIIIA/IRE-MS2 and pAD-IRP plasmid vector质粒载体

pIIIA/IRE-MS2 and pAD-IRP. Plasmid pIIIA/IRE-MS2 expresses 5' IRE-MS2 3' Hybrid RNA from the yeast RNAseP promoter, and is on a multi-copy URA3 plasmid. Plasmid pAD-IRP expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain. The plasmid is multi-copy and carries a LEU2 marker. The parent vector for this plasmid is pACTII, constructed by Steve Elledge. pIIIA/IRE-MS2 and pAD-IRP, introduced into strain L40-coat, cause transcription activation of HIS3 and LacZ. If you want to assay for HIS3 transcriptional activation, include 3-aminotriazole to eliminate low levels of basal expression. For this combination of plasmids (pIIIA/IRE-MS2 and pAD-IRP), plate our transformants directly onto 5 mM 3-aminotriazole

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