pACTII plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pACTII plasmid vector质粒载体

pACTII. This plasmid (constructed by Steve Elledge's laboratory) can be used to create a hybrid protein in which a polypeptide of interest is linked to the GAL4 Activation Domain. pACTII carries the GAL4 Activation Domain followed by an HA epitope tag and a polylinker for cloning the gene of interst (see enclosed map for restriction sites). The HA epitope permits immunodetection of the hybrid protein. The hybrid protein is expressed from the ADH promoter. This multicopy plasmid carries a LEU2 marker.

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