E.coli BL21 Star (DE3) strain /Competent cells大肠杆菌菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

E.coli BL21 Star (DE3) strain /Competent cells大肠杆菌菌株/感受态细胞

Description
E. coli  BL21 Star (DE3) is designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., pET vectors).

• Contain a genotype that promotes high mRNA stability and protein yield
• Optimized for use with low copy number, T7 promoter-based plasmids
• Provided in a single-tube, single-use format—allows all transformation steps to take place in the same tube, thereby helping to save time and prevent contamination

Enhanced expression of nontoxic recombinant proteins
BL21 Star (DE3) cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter. IPTG is required to induce expression. This strainalso offers enhanced mRNA stability due to a mutation in the RNaseE gene (rne131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. Protein expression is further enhanced by the absence of thelon and outer membrane (OmpT) proteases, which reduces degradation of heterologous proteins.

Note: The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes.

Genotype:F-ompT hsdSB (rB-, mB-) galdcmrne131 (DE3)

Supplier来源:BioVector NTCC Inc.
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