Ruminococcus bromii strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Ruminococcus bromii strain菌株

Ruminococcus bromii Moore et al.
NTCC®027255

Ruminococcus bromii strain VPI 6883 is a whole-genome sequenced bacterial type strain that was isolated from human feces.
Product category
Bacteria
Product type
Anaerobe
Strain designation
VPI 6883
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Feces
Product format
Frozen
Storage conditions
-80°C or colder

Characteristics
Comments
Grows best when using Anaerobe Systems PYG broth.
Handling information
Temperature
37°C
Atmosphere
Anaerobic
Handling procedure
Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.
Under anaerobic conditions aseptically transfer the entire contents to a 5-6 mL tube of Anaerobe Systems PYG broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary broth tubes. Best practice dictates the use of pre-reduced media.
Use several drops of the primary broth tube to inoculate a Brucella Blood plate.
Incubate in an anaerobic atmosphere at 37°C for 48 to 72 hours. Incubate one agar plate aerobically at 37°C to check for contamination.

ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by the use of an anaerobic gas chamber or placement of test tubes under a gassing cannula system connected to anaerobic gas.

Anaerobic conditions for incubation may be obtained by any of the following:
Loose screw caps on test tubes in an anaerobic chamber
Loose screw caps on test tubes in an activated anaerobic gas pack jar
Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained

Handling notes
Grows best when using Anaerobe Systems PYG broth.
This culture is sensitive to oxygen when initially rehydrated; therefore steps should be taken to avoid exposure to oxygen. When the culture exhibits good growth it will remain viable for up to 1 week if stored at 4°C under anaerobic conditions.
When examined microscopically, the cells appear as cocci generally in chains. Motility was not detected. No growth occurred on the non-selective media.
The following sequences of this organism is available in GenBank:

AX110950 Sequence 1683 from Patent WO0123604.
L76600 Ruminococcus bromii small subunit ribosomal RNA (16S rDNA) gene.


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