pSR657 plasmid vector昆虫细胞表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSR657 plasmid vector昆虫细胞表达质粒载体

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells
Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824.

VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511.

BACKBONE
Vector backbonepFastBac-Dual
Backbone manufacturerInvitrogen
Backbone size w/o insert (bp)5238
Total vector size (bp)9335
Vector typeInsect Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Temperature37°C
Growth Strain(s)DH10B
Copy numberLow Copy
GENE/INSERT 1
Gene/Insert namemodified AAV-2 rep78
Insert Size (bp)1866
Promoterpolyhedrin
CLONING INFORMATION FOR GENE/INSERT 1
Cloning methodRestriction Enzyme
5′ cloning siteBamHI (destroyed during cloning)
3′ cloning siteXbaI (not destroyed)
5′ sequencing primerAAATGATAACCATCTCGC
3′ sequencing primerGAAATTTGTGATGCTATTGC
(Common Sequencing Primers)
GENE/INSERT 2
Gene/Insert namemodified AAV-2 cap
Insert Size (bp)2208
Promoterp10
CLONING INFORMATION FOR GENE/INSERT 2
Cloning methodRestriction Enzyme
5′ cloning siteNheI (not destroyed)
3′ cloning siteXmaI (not destroyed)
5′ sequencing primerACGGACCTTTAATTCAACCCA
3′ sequencing primerCTTCCGTGTTTCAGTTAGC

Supplier来源:BioVector NTCC Inc.
TEL电话:400-800-2947
Website网址: http://www.biovector.net