Yarrowia lipolytica Po1g strain菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Yarrowia lipolytica Po1g strain菌株

NTCC® Yarrowia lipolytica Po1g strain is a derivative of the wild-type strain W29 (ATCC 20460) by a series of genetic modifications. Deletion of the AXP gene, encoding the acidic protease, gave rise to strain Po1f. Po1e and Po1g were obtained by transformation of Po1d and Po1f, respectively, with a pBR322-URA3 vector, forming a docking platform to direct integration of the pBR322-based expression /secretion vectors.

Phenotype: Leu−, ΔAEP, ΔAXP, Suc+, pBR
Genotype: MatA, leu2-270, ura3-302::URA3, xpr2-322, axp-2

The cells were maintained on a sterilized yeast-, peptone-, and dextrose- (YPD-) agar plate containing 10 g/L yeast extract (Bacto, France), 10 g/L peptone (Bacto, France), 20 g/L glucose (Acros Organics, USA), and 20 g/L agar (Acros Organics, USA) at 4°C for further microbial fermentation.

Application transformation host
Medium YPD Medium
Broth Medium
YPD Broth(BD 242820)……………………....50 g
DI Water………………………………………..1000 ml
Or for solid medium:
Agar Medium
YPD Agar(BD 242720)………………………..65 g
DI Water………………………………………...1000 ml
Dispense into appropriate vessel and autoclave at 121ºC.
*YPD is commercially available from BD. To make medium from scratch, follow
formulation below:
Yeast Extract……………………………………10.0 g
Peptone…………………………………………20.0 g
Dextrose………………………………………...20.0 g
Agar (if needed)………………………………..20.0 g
DI Water………………………………………...1000 ml
Adjust for final pH of 5.6. Autoclave at 121oC.
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic


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