BioVector® GB05-dir E.coli strain大肠杆菌菌株/感受态细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

BioVector® GB05-dir E.coli strain大肠杆菌菌株/感受态细胞

Red/ET recombination relies on homologous recombination in vivo in E.coli and
allows a wide range of modifications on DNA molecules of any size and at any
chosen position. Homologous recombination is the exchange of genetic material
between two DNA molecules in a precise, specific and accurate manner.
Homologous recombination occurs through homology regions, which are stretches of
DNA shared by the two molecules that recombine. Because the sequence of the
homology regions can be chosen freely, any position on a target molecule can be
specifically altered. Red/ET recombination allows you to choose homology arms as
short as 50 bp for homologous recombination, which can easily be added to a
functional cassette by long PCR primers.
Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient
linear-to-linear homologous recombination mechanistically distinct from conventional
recombineering mediated by Redαβ from lambda phage (Fu et al. 2012).
E. coli strain BioVector® GB05-dir harbours an arabinose inducible ETgA operon (full length
recE, recT, redγ, and recA) at the ybcC locus. The strain was successfully used to
directly clone gene clusters larger than 10 kb of secondary-metabolite pathways from
Photorhabdus luminescens, a gram negative rod-shaped bacterium, into expression
vectors (Fu et al. 2012).

1. BioVector® GB05-dir: Glycerol stock of recombineering proficient E. coli strain GB05-dir
(500 µl, 25% glycerol)

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