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pFastBacHT C质粒载体图谱序列抗性价格报价Biovector NTCC Inc.现货

  • 价  格:¥1920
  • 货  号:BiovectorpFastBacHT C
  • 产  地:北京
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pFastBacHTC载体基本信息

出品公司:Invitrogen
载体名称:pFastBacHTC, pFastBac HTC, pFastBacHT C, pFastBac HT C
质粒类型:昆虫细胞表达载体
启动子:Polyhedrin
表达水平:
克隆方法:多克隆位点,限制性内切酶
载体大小:4858 bp
5' 测序引物:pFastbac-F: TATTCCGGATTATTCATACC
3' 测序引物:pFastBac-R: ACAAATGTGGTATGGCTGA
载体标签:N-His, N-TEV
载体抗性:Ampicillin 氨苄
筛选标记:Gentamicin 庆大霉素
备注:
产品目录号:10584-027
稳定性:稳表达 Stable
组成型:组成型 Constitutive
病毒/非病毒:杆状病毒

pFastBacHTC载体质粒图谱和多克隆位点信息

pFastBac HTC载体图谱

pFastBac HTC多克隆位点

pFastBacHTC载体简介

The pFastBac HT vector is supplied with the multiple cloning site in three reading frames (A, B, and C) to facilitate cloning your gene of interest in frame with the N-terminal 6xHis tag.

• Strong polyhedrin (PH) promoter for high-level protein expression.
• N-terminal 6xHis tag for purification of recombinant fusion proteins using metal-chelating resin and a TEV protease cleavage site for removal of the 6xHis tagfollowing protein purification.
• Vector supplied in 3 reading frames for simplified cloning.

pFastBacHTC载体序列

LOCUS       pFastBacTHT\C           4858 bp    DNA     circular SYN  2-SEP-2003
SOURCE      
 ORGANISM  
COMMENT     This file is created by Vector NTI
           http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
    source          1..4858
                    /invitrogen="608"
                    /vntifkey="98"
    CDS             589..1449
                    /invitrogen="1020000"
                    /vntifkey="4"
                    /label=Amp(R)
    promoter        490..588
                    /invitrogen="2330000"
                    /vntifkey="30"
                    /label=bla\promoter
    rep_origin      2..457
                    /invitrogen="2520000"
                    /vntifkey="33"
                    /label=f1\origin
    CDS             complement(2802..3335)
                    /invitrogen="1150000"
                    /vntifkey="4"
                    /label=Gm(R)
    misc_feature    4119..4224
                    /invitrogen="1550000"
                    /vntifkey="21"
                    /label=MCS
    promoter        complement(3524..3551)
                    /invitrogen="2250000"
                    /vntifkey="29"
                    /label=Pc\promoter
    promoter        3904..4032
                    /invitrogen="2260000"
                    /vntifkey="29"
                    /label=PH\promoter
    primer          3920..3937
                    /invitrogen="2060000"
                    /vntifkey="27"
                    /label=Polyhedrin\forward\primer
    rep_origin      1594..2267
                    /invitrogen="2540000"
                    /vntifkey="33"
                    /label=pUC\origin
    polyA_signal    4242..4482
                    /invitrogen="1850000"
                    /vntifkey="25"
                    /label=SV40\pA
    CDS             4101..4121
                    /invitrogen="1300000"
                    /vntifkey="4"
                    /label=TEV\recognition\site
    misc_recomb     4501..4695
                    /invitrogen="2750000"
                    /vntifkey="86"
                    /label=Tn7L
    misc_recomb     2512..2735
                    /invitrogen="2760000"
                    /vntifkey="86"
                    /label=Tn7R
    CDS             4062..4079
                    /invitrogen="1015000"
                    /vntifkey="4"
                    /label=6xHis
    CDS             4050..4052
                    /invitrogen="123000000"
                    /vntifkey="4"
                    /label=initiation\ATG
BASE COUNT     1219 a      1198 c      1153 g      1288 t
ORIGIN
       1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
     481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
    2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
    2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
    2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
    2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
    2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
    2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
    2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
    2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
    2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
    2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
    2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
    3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
    3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
    3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
    3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
    3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
    3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
    3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
    3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
    3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
    3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
    3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
    3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
    3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
    3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
    3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
    3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
    3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
    4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
    4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatgggg atccggaatt
    4141 caaaggccta cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt
    4201 ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga ggatcataat cagccatacc
    4261 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
    4321 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
    4381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    4441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact agatctgcct
    4501 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt
    4561 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa
    4621 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg
    4681 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
    4741 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
    4801 attaatgttt gtaattgact gaatatcaac gcttatttgc agcctgaatg gcgaatgg


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