pYUB854分枝杆菌基因等位交换载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pYUB854 plasmid vector质粒载体

BioVector NTCC质粒载体菌种细胞基因保藏中心

pYUB854. HygR; allelic exchange vector. Cosmid vector, with res sites flanking the HYGR gene.

Cosmid vector pYUB572 is a derivative of pYUB328 (Balasubramanian et al., 1996), in which 2±2 kb fragment containing one of the λ-cos sites was removed by cleavage with MunI and Csp45I.

Cosmid pYUB854 is a derivative of pYUB572, in which the bla gene was removed by digestion with BspHI and replaced with a res–hyg–res gene cassette flanked by multiple cloning sites (MCSs).

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