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pET Systems原核表达系统-BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥2930
  • 货  号:pET Systems
  • 产  地:北京
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BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

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地址:北京

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pET Systems

Efficiently clone and express your target protein by cloning your gene into one of our industry leading bacterial, mammalian or insect cell systems. EMD Millipore’s vast portfolio of expression vectors enables you to choose the perfect combination of promoters, epitope tags, antibiotic resistance, and host compatibility.
pET E. coli T7 Expression Vectors
The pET System is the most powerful system for the cloning and expression of recombinant proteins in E. coli. Driven by the strong bacteriophage T7 promoter and translation signals, Novagen’s® pET System has been used to express thousands of different proteins in host cells expressing T7 polymerase. The pET System has continuously expanded to offer new technologies and options for expression. Collectively, our large collection of pET vector types, different host strains and companion products offer complete solutions for high level expression, purification and detection of target proteins:

Browse all pET Vectors

Popular Novagen pET vectors
Unique Protein Expression Features
Vector of Choice
high yield bioproduction of peptides and small proteinspET-31b(+)
production of soluble, active target proteins in E. coli

pET-32a(+), pET-32b(+), pET-32c(+)

production of target proteins suitable for site-specific 32P-labelingpET-33b(+)
Dsb tags for export and periplasmic folding of target proteins

pET-39b(+), pET-40b(+)

with popular GST fusion tags for enhanced production and solubility

pET-41a(+), pET-41b(+), pET-41c(+) ,pET-42a(+), pET-42b(+), pET-42c(+)

designed for cloning and high-level expression of polypeptide sequences fused with the 495 aa NusA (Nus•Tag™) protein

pET-43.1a(+), pET-43.1b(+), pET-43.1c(+)

with Nus•Tag™ sequence plus N- and C-terminal His•Tag sequences

pET-44a(+), pET-44b(+), pET-44c(+)

with amino-terminal His•Tag™ sequence and minimal extraneous sequencespET-45b(+)
prepared vector for ligation-independent cloning, with amino-terminal His•Tag™ sequencepET-46 Ek/LIC
with HRV 3C Protease cleavage site for efficient fusion tag removal

pET-47b(+), pET-48b(+), pET-49b(+), pET-50b(+)


Nus•Tag™ fusion increases the solubility of recombinant annexin A1.

Nus•Tag™ fusion increases the solubility of recombinant annexin A1.
pENTR™ vectors containing the annexin A1 target gene were generated using the Gateway Nova pET-53-DEST™ vector (lanes 2-3, pET-His•Tag®/annexin-A1/Strep•Tag® II) and Gateway Nova pET-57-DEST vector (lanes 4-5, pET-His•Tag/Nus•Tag/thrombin/annexin-A1/Strep•Tag II).

Soluble annexin A1 was purified on sequential His•Bind and Strep•Tactin columns (lanes 6-7). The N-terminal fusion tags were removed by thrombin cleavage and a subtractive His•Bind column (lanes 8-9).


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