首页 » MDA-MB-231/ Taxol人乳腺癌细胞紫杉醇耐药株BioVector NTCC质粒载体菌种细胞基因保藏中心

MDA-MB-231/ Taxol人乳腺癌细胞紫杉醇耐药株BioVector NTCC质粒载体菌种细胞基因保藏中心

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  • 货  号:MDA-MB-231/ Taxol
  • 产  地:北京
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MDA-MB-231/ Taxol Cell Line 人乳腺癌细胞株紫杉醇耐药株

-BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心




Catalog Number:



NTCC594202



Growth Properties
   :



adherent



Organism :



Homo sapiens (human)



Source :



Organ: mammary
   gland/breast

Cell type:
epithelial-like

Disease: adenocarcinoma



Comments :



The
MDA-MB-231 human breast cancer cell line was established
   from tumor tissue from an untreated patient.



Propagation :



Complete growth medium:RPMI 1640 medium,
   90%; fetal bovine serum, 10%

Temperature:37.0
,5%CO2



Drug
   resistance:



The
   highest concentration of Taxol resistance is 30ng/ml.



Subculturing:




Protocol: Remove
   medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the
   solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the
   flask to sit at room temperature (or at 37
) until the cells detach. Add fresh culture
   medium, aspirate and dispense into new culture flasks.

Subcultivation ratio:A
   subcultivation ratio of 1:2 to 1:4 is recommended

Medium renewal:Every 2 to
   3 days



Preservation :



Freeze medium:Complete growth
   medium, 95%; DMSO, 5%

Storage temperature:Liquid
   nitrogen vapor temperature




















































中文名称: 人乳腺癌紫杉醇耐药株

形态学: 上皮细胞

生长方式: 贴壁

培养条件:

培养基: RPMI1640  

血清: 胎牛血清    

耐药浓度: 30ng/ml



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