DMS273 cell line人小细胞肺癌胸膜液标本细胞株

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

CatalogueNo.:NTCC®-95062830

CellLineName:DMS273

CellLineDescription:DMS273 was initiated in 1978 from the pleural fluid specimen from a 50

year old female patient withsmall cell carcinoma of the lung. The patient was in relapse after chemotherapyand radiotherapy. DMS 273 cells exhibit intercellular processes and expressretinoblastoma mRNA and protein. The cell line is tumorigenic in nude mice.

GeneralInfo

Species:

Human

Characteristics

TissueofOrigin:

Pleuralfluid

DNAprofile(STRProfile):

Amelogenin: X CSF1PO:10,12

D5S818:11,12

D7S820:10,12

D13S317:12,13

D16S539: 11

TH01:9.3

TPOX:10

vWA:15

Karyotype:Notspecified

Applications:Humanlungtumourmodel

Disease:SmallCellCancer

CultureConditions

Subculture Routine:

Split sub-confluent cultures(70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.05% trypsin ortrypsin/EDTA; 5% CO₂; 37°C.

CultureMedium:

Waymouth's MB 752/1 + 2mMGlutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

GrowthMode:Adherent

CountryofOrigin:UnitedStates

HazardGroup(ACDP):HazardGroup(ACDP)2

Images

AvailableFormats:

Frozen

DNA-5µg(100ng/µl)

Citation Guidance

Ifuseofthiscultureresultsinascientificpublication,itshouldbecitedinthepublicationas:DMS273(NTCC®-95062830).

Biosafety Information 2

Copyright©BioVector NTCC Inc.

Name细胞名称：NTCC® DMS273 Lung Small Cell Lung Cancer Cell Line
Description描述：Lung Lung Neuroendocrine Tumor Small Cell Lung Cancer cell line细胞株
ID编号:NTCC®-ACH-000749
Category分类：Small Cell Lung Cancer Cell Line
Size/Quantity数量: 1 Flask/2 Vials
Hazard Group (ACDP)生物安全级别:2
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Species物种来源: Lung
Age年龄:
Gender性别:
Cell Type细胞类型: Lung Neuroendocrine Tumor
Cell Images细胞图片:
Complete Growth Medium完全培养基: NTCC® DMS273 Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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