HUDEP-2 Cell Line NTCC®源自脐带血细胞的人红系祖细胞系。表达成人型血红蛋白。细胞株-BioVector细胞库RCB4557

Name细胞名称: NTCC® HUDEP-2 Cell Line 源自脐带血细胞的人红系祖细胞系。表达成人型血红蛋白。
Description描述：Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.源自脐带血细胞的人红系祖细胞系。表达成人型血红蛋白。
ID编号:NTCC®-924557
Category分类：Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Species物种来源:
Age年龄:
Gender性别:
Cell Type细胞类型: Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.
Cell Images细胞图片:
Complete Growth Medium完全培养基: BioVector® HUDEP-2 Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

HUDEP-2 cell line来源于脐带血细胞的人红细胞祖细胞株，表达成人型血红蛋白

BioVector NTCC质粒载体菌株细胞蛋白抗体基因保藏中心

Comment:

Human erythroid progenitor cell line derived from umbilical cord blood cells. Expressing adult type hemoglobin.
Images:

Year of deposit 2013
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Tissue umbilical cord blood
Classification transformed
Recombinant recombinant
Exogene KUSABIRA-ORANGE, HPV16 E6/E7
Vector CSIV-TRE-HPV-E6/E7-UbC-KT lentiviral vector
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_VI06
deposit info
lot info
Medium Medium List
Culture type Suspension cells Suspension cells
Medium and additives StemSpan SFEM (Stemcell technologies), EPO(3U/ml), SCF(50ng/ml), DEX(10-6M), DOX(1ug/ml) StemSpan SFEM +3U/ml EPO + 50ng/ml SCF + 10^(-6)M Dexamethasone + 1ug/ml Doxycycline
Antibiotics  Free
Passage method  dilution
Culture information Passage ratio  1 : 5-10 split
SC frequency Subculture : 2 times/week Subculture : 2 times/week
Temperature 37 ℃ 37 ℃
CO2 concentration 5 % 5 %
Freeze medium Medium + 10% DMSO
Freezing method  Slow freezing
Mycoplasma  (-)
Animal PCR  OK
Virus (HIV)  Undetected
Virus (HTLV-1)  (-)
STR(human)  OK

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net