TOp2380 出芽酵母载体质粒BioVector NTCC质粒载体菌种细胞基因保藏中心Budding yeast vector

TOp2380  【载体类型Vector Type】： 【插入基因Inserted Gene】： 2×RUB1(Δintron)  【大肠杆菌筛选标记E.coli selection marker】： Amp  【酵母筛选标记S.pombe selection marker】： 【其他Comments】: (I) PCR(RUB1-1, RUB1-3, W303D) Klenow, PCR (RUB1-2, RUB1-4, W303D) Klenow, , (II) PCR (RUB1-1, RUB1-2, (I)PCR product 2 species)BamHI, XhoI, (III) PCR (RUB1-5, RUB1-6, (II)PCR product cut-off fragment) EcoRI, BamHI, L. pKT10GAL (EcoRI-XhoI), (III) PCR-(II) PCR- pKT10GAL, 2×RUB1(Δintron)-pKT10GAL