KYO-1原细胞危象中的慢性粒细胞白血病细胞株chronicmyeloid leukemia in blast crisis NTCC® Cancer cell line-BioVector NTCC细胞库

[Designation名称] NTCC® ID   KYO-1 Cell Line细胞株
BioVector NTCC Inc.

Species:

human (Homosapiens)

Cell type:

chronicmyeloid leukemia in blast crisis

Origin:

establishedfrom the peripheral blood of a 22-year-old man with chronic myeloid leukemia(CML) in myeloid blast crisis in 1981; cells carry Philadelphia-chromosometranslocation

Biosafetylevel:

1

NTCC® CellCulture Data:

Morphology:

round cellsgrowing mostly singly or occasionally in clumps in suspension; a few cells aredouble-sized;

Images:

Medium:

80-90% RPMI1640 + 10-20% h.i. FBS

Subculture:

seed out atca. 0.5 x 10⁶ cells/ml; split culture 1:2 to 1:4 every 2-3days; maintain at ca. 0.2-0.8 x 10⁶ cells/ml

Incubation:

at 37 °C with5% CO₂

Doubling time:

ca. 40-50hours

Harvest:

cell harvestof ca. 1 x 10⁶ cells/ml

Storage:

frozen with70% medium, 20% FBS, 10% DMSO

NTCC®Scientific Data:

Mycoplasma:

negative inmicrobiological culture, PCR assays

Immunology:

CD3-, CD11b-,CD13-, CD14-, CD15-, CD19-, CD33+, CD34-, CD41-, CD42-, CD71+;

Authenticity:

STR analysisaccording to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted inan authentic STR profile of the reference STR database.

Cytogenetics:

humanflat-moded hypotetraploid karyotype; 83-90<4n>XX, -Y, -Y, +5, +8, -9, -9,+11, +11, +15, -16, -17, -17, -18, -18, +22, +22, +2mar; t(3;12)(p12.2;p11-12)x2, der(5)t(5;6)(p15;?q22)t(6;12)(q2?;?)ins(6;12)(q2?;?), del(6)(q22) x2,der(8)t(8;9)(q25;p11-12) x2, der(9)add(9)(p11-12)t(9;22)(q34;q11) x2,add(11)(q11) x2, der(12)t(6;12)(?;?) x2, der(15)t(15;22)(p11;q11) x2,der(17)t(17;17)(p12-13;q23) x2, der(22)t(9;22)(q34;q11) x4; carries t(9;22)effecting BCR-ABL1 rearrangement; resembles published karyotype

Molec.Genetics:

expression offusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR

Viruses:

PCR: EBV -,HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -

DR   ArrayExpress; E-MTAB-2770
DR   BioSample; SAMN03473497
SY   KYO1; Kyoto 1
DR   BioSample; SAMN10988458
DR   cancercelllines; CVCL_2095
DR   Cell_Model_Passport; SIDM01471
DR   CGH-DB; 9209-4
DR   Cosmic; 787499
DR   Cosmic; 1026567
DR   DepMap; ACH-000604
DR   DSMZ; ACC-601
DR   DSMZCellDive; ACC-601
DR   GEO; GSM887246
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC   CVCL_2095
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID   KYO-1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net