GM04978 MUSCULAR DYSTROPHY, DUCHENNE TYPE; DMD Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® GM04978 MUSCULAR DYSTROPHY, DUCHENNE TYPE; DMD Cell Line
杜兴型肌营养不良症； DMD细胞株
Description描述：Clinically affected; pseudohypertrophy of calves; severe proximal muscle weakness; positive Gower's sign; muscle biopsy shows advanced dystrophic process; elevated serum CPK of 13,020; affected brother and maternal male first cousin; mother is GM04982; dystrophin gene shows no detectable deletion or duplication by multiplex ligation probe amplification (MLPA) analysis; DNA sequencing showed this donor has a C>T transition at nucleotide 5893 of the DMD gene (c.5893C>T) resulting in a stop at codon 1965 [Gln1965Ter (Q1965X)]
Category分类：Fibroblast
ID编号:NTCC®-GM04978
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race: Black/African American
Age年龄:13 YR
Gender性别:Male
Cell Type细胞类型: MUSCULAR DYSTROPHY, DUCHENNE TYPE; DMD
Affected State:Yes
Product:Fibroblast
Gene:DMD
Mutations突变:GLN1965TER
Ethnicity:
Family:805
Relationship:proband
Karyotype:
Tissue Type组织来源:
Complete Growth Medium完全培养基: BioVector® Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net