AG08082 EQUUS | ORDER: PERISSODACTYLA Aorta, thoracic Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG08082 EQUUS | ORDER: PERISSODACTYLA Aorta, thoracic Cell Line
雅科仕 |目： 奇蹄目细胞株
Description描述：Quarter horse breed. The thoracic aorta was removed post-mortem on 3/21/85. Strips of the medial layer were removed, minced and used as explants to initiate the culture. Cell morphology is fibroblast-like and the cells form layered sheets at post-confluence. The karyotype is 64,XY; normal diploid male. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured in Ham's F12 medium and MCDB104 medium in a 1/1 mixture with 10% inactivated fetal bovine serum and supplements (0.04-0.06mg/ml crude ECGF and 0.05mg/ml heparin). A skin fibroblast culture from this horse is AG08080. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Smooth muscle
ID编号:NTCC®-AG08082
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:16 YR
Gender性别:Male
Cell Type细胞类型: EQUUS | ORDER: PERISSODACTYLA
Affected State:No
Product:Smooth muscle
Gene:
Mutations突变:
Ethnicity:
Family:1096
Relationship:proband
Karyotype:64,XY
Tissue Type组织来源:Artery
Complete Growth Medium完全培养基: BioVector® Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
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