AG08503 BOS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line NTCC®细胞株-BioVector NTCC细胞库

Name细胞名称：NTCC® AG08503 BOS | ORDER: CETARTIODACTYLA Aorta, thoracic Cell Line
博斯|目：鲸齿兽细胞株
Description描述：Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/22/85. The culture was initiated from cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The first and second passage cells were propagated in medium supplemented with horse plasma-derived serum and heparin (0.1mg/ ml) on gelatin-coated flasks. The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: AMMC 20U with supplements. For lifespan determination this cell line was cultured in Ham's F12 medium with 10% inactivated fetal bovine serum. A smooth muscle cell culture from this calf is AG08504A. This cell line will only be shipped frozen. The legacy karyotype description shown in this Remark may not be representative of the current available product.
Category分类：Endothelial
ID编号:NTCC®-AG08503
Size/Quantity数量: 1 Flask/2 Vials
Biosafety Level生物安全级别:1
Shipping Info运输方式: RT/Dry Ice
Storage储存方式: RT/Liquid Nitrogen
Race:
Age年龄:1 YR
Gender性别:Male
Cell Type细胞类型: BOS | ORDER: CETARTIODACTYLA
Affected State:No
Product:Endothelial
Gene:
Mutations突变:
Ethnicity:
Family:2059
Relationship:proband
Karyotype:60,XY
Tissue Type组织来源:Artery
Complete Growth Medium完全培养基: BioVector® MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent Complete Medium
Subculturing传代方法:
Cryopreservation冻存方法:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions培养条件:Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
STR Profile鉴定数据:
References参考文献:

Supplier供应商:BioVector质粒载体菌株细胞蛋白抗体基因保藏中心
NTCC典型培养物保藏中心
Tel电话：400-800-2947
Email：Biovector@163.com
http://www.biovector.net