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MC38-GFP绿色荧光稳定表达细胞株
Description: MC38 cell line
Organism:Mus musculus (mouse) , C57BL6 murine
Organ:colon
Disease:colorectal adenocarcinoma
CellType: epithelial-like
GrowthProperties: adherent
Medium:Dulbecco's modified MEM with 10% fetal bovine serum, 2mM glutamine, 0.1 mMnonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50ug/ml gentamycinsulfate, pen/strep.
Tumorigenic:Yes
Subculturing Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution toremove all traces of serum that contains trypsin inhibitor.
3. Add2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under aninverted microscope until cell layer is dispersed (usually within 5 minutes).
Note:To avoid clumping do not agitate the cells by hitting or shaking the flaskwhile waiting for the cells to detach. Cells that are difficult to detach maybe placed at 37°C to facilitate dispersal.
4. Add6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Addappropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.
SubcultivationRatio: A subcultivation ratio of 1:5 to1:10 is recommended
Medium Renewal: 2to 3 times per week
Freeze medium: Completegrowth medium 95%; DMSO, 5%
Storagetemperature: liquid nitrogen vapor phase
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