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MC38-GFP绿色荧光稳定表达细胞株-NTCC保藏中心

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  • 货  号:MC38-GFP绿色荧光稳定表达细胞株-NTCC保藏中心
  • 产  地:北京
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MC38-GFP绿色荧光稳定表达细胞株


Description: MC38 cell line


Organism:Mus musculus (mouse) , C57BL6 murine

Organ:colon

Disease:colorectal adenocarcinoma

CellType: epithelial-like

GrowthProperties: adherent

Medium:Dulbecco's modified MEM with 10% fetal bovine serum, 2mM glutamine, 0.1 mMnonessential amino acids, 1 mM sodium pyruvate, 10 mM Hepes, 50ug/ml gentamycinsulfate, pen/strep.

Tumorigenic:Yes

Subculturing Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution toremove all traces of serum that contains trypsin inhibitor.

3. Add2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under aninverted microscope until cell layer is dispersed (usually within 5 minutes).

Note:To avoid clumping do not agitate the cells by hitting or shaking the flaskwhile waiting for the cells to detach. Cells that are difficult to detach maybe placed at 37°C to facilitate dispersal.

4. Add6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5. Addappropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

SubcultivationRatio: A subcultivation ratio of 1:5 to1:10 is recommended

Medium Renewal: 2to 3 times per week

Freeze medium: Completegrowth medium 95%; DMSO, 5%

Storagetemperature: liquid nitrogen vapor phase



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