BU3 NKX2-1-GFP SFTPC-tdTomato人类诱导多能干细胞NTCC® Human Induced pluripotent stem cell细胞系-BioVector NTCC细胞保藏中心CVCL_WN82

[Designation名称] NTCC® BU3 NKX2-1-GFP SFTPC-tdTomato Cell Line细胞株
BioVector NTCC典型培养物保藏中心

SY BU3 NKX2-1GFP;SFTPCtdTomato; BU3-NGST; BU3 NGST; BU3NGST
DR Wikidata; Q93433163
RX PubMed=28965766;
RX PubMed=31924806;
WW https://stemcellbank.bu.edu/Catalog/Item/Details/509
CC From: Boston University School of Medicine; Boston; USA.
CC Population: Caucasian.
CC Characteristics: Using CRISPR/Cas9 a 2A-eGFP-pA was inserted after the NKX2-1 coding sequence and using TALENs a tdTomato-pA-loxP-PGKp-PuroR-pA-loxP was inserted at the SFTPC translational start site.
CC Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC Genetic integration: Method=TALEN; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC Genetic integration: Method=TALEN; Gene=UniProtKB; P13249; S.alboniger pac (PuroR).
CC Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_WN82
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® BU3 NKX2-1-GFP SFTPC-tdTomato Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net