CALM/AF10 Cas9小鼠癌细胞株NTCC® Mouse Cancer cell line细胞系-BioVector NTCC细胞保藏中心CVCL_C8ZE

[Designation名称] NTCC® CALM/AF10 Cas9 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

SY Line #171
DR BioGRID_ORCS_Cell_line; 815
DR Wikidata; Q123030850
RX PubMed=29478914;
CC Characteristics: The CALM/AF10 oncogene was transduced into bone marrow hematopoietic stem cells and then transferred cells to sub-lethally irradiated recipients. Primary acute myeloid leukemia cells were harvested 3-6 months later, serially transplanted three times and then cultured in the presence of cytokines (PubMed=29478914).
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC Omics: CRISPR phenotypic screen.
DI NCIt; C21604; Mouse leukemia
OX NCBI_TaxID=10090; ! Mus musculus (Mouse)
SX Male
CA Cancer cell line
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C8ZE
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® CALM/AF10 Cas9 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net