CAMA-1/Cas9-hyg人类癌细胞株NTCC® Human Cancer cell line细胞系-BioVector NTCC细胞保藏中心CVCL_C9DC

[Designation名称] NTCC® CAMA-1/Cas9-hyg Cell Line细胞株
BioVector NTCC典型培养物保藏中心

DR GeneCopoeia; SL562
DR Wikidata; Q123030852
CC Population: Caucasian.
CC Characteristics: Stably expresses a EF1a-Cas9-HA-SV40-EGFP-IRES-HygR construct (GeneCopoeia=SL562).
CC Sequence variation: Mutation; HGNC; 1748; CDH1; Simple; c.1712-1G>A; Zygosity=Homozygous; Note=Splice acceptor mutation (from parent cell line).
CC Sequence variation: Mutation; HGNC; 9588; PTEN; Simple; p.Asp92His (c.274G>C); Zygosity=Heterozygous (from parent cell line).
CC Sequence variation: Mutation; HGNC; 11998; TP53; Simple; p.Arg280Thr (c.839G>C); ClinVar=VCV000012368; Zygosity=Homozygous (from parent cell line).
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
CC Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
CC Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
DI NCIt; C5214; Breast adenocarcinoma
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C9DC
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[Reference参考文献]
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培养方法：
Medium培养基：NTCC® CAMA-1/Cas9-hyg Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net