CCMB-Me-14-STGG-A1小鼠胚胎干细胞NTCC® Mouse Embryonic stem cell细胞系-BioVector NTCC细胞保藏中心CVCL_C4SE

[Designation名称] NTCC® CCMB-Me-14-STGG-A1 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

SY STGG
DR Wikidata; Q116048697
RX PubMed=36063726;
CC Characteristics: Using CRISPR/Cas9 a T2A-H2B-tdTomato construct was inserted at the C-terminus of one allele of Sox2 and a H2B-GFP-SV40-Bsd cassette was inserted at the C-terminus of one allele of Gata6 (PubMed=36063726).
CC Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
CC Genetic integration: Method=CRISPR/Cas9; Gene=HGNC; 4761; H2BC11.
CC Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
CC Genetic integration: Method=CRISPR/Cas9; Gene=UniProtKB; P0C2P0; Aspergillus terreus blasticidin-S deaminase (Bsd).
CC Genetic integration: Method=CRISPR/Cas9; Gene=FPbase; PGG5S; tdTomato (Note=Bright red fluorescent protein derived from drFP583 (DsRed)).
CC Derived from site: In situ; Blastocyst; UBERON=UBERON_0000358.
CC Cell type: Embryonic stem cell; CL=CL_0002322.
CC Breed/subspecies: 129P2/Ola.
[Organism物种来源]
[Disease疾病种类]
[Accession编号] NTCC®-AC CVCL_C4SE
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® CCMB-Me-14-STGG-A1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Website网址：www.biovector.net