CHO C1s-/- MGAT1- BaL-rgp120中国仓鼠自发永生化细胞株NTCC® Chinese hamster Spontaneously immortalized cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID CHO C1s-/- MGAT1- BaL-rgp120 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: BaL-rgp120 C1s-/- MGAT1- CHO
Cross-references: BEI_Resources; ARP-13573
Cross-references: Wikidata; Q108820027
References identifiers: PubMed=31087560;
Comments: Group: Serum/protein free medium cell line.
Comments: Characteristics: Produces an unclipped gp120 from a clade B isolate (BaL-rgp120) and enriched for mannose-5 glycans. The gp120 construct contains an N-terminal purification tag from HSV-1 glycoprotein D (gD) (PubMed=31087560).
Comments: Knockout cell: Method=CRISPR/Cas9; UniProtKB; Q924C0; Mgat1.
Comments: Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GUR0; C1s.
Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; Q78225; HIV-1 isolate BaL env.
Comments: Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments: Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Comments: Cell type: Epithelial cell of ovary; CL=CL_2000064.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_A9NZ
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID CHO C1s-/- MGAT1- BaL-rgp120 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

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