CHO-K1 UA10中国仓鼠自发永生化细胞株NTCC® Chinese hamster Spontaneously immortalized cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID CHO-K1 UA10 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: UA10
Cross-references: Wikidata; Q98125620
References identifiers: PubMed=32346859;
Comments: Characteristics: Doubly auxotrophic; deficient in 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC) and uridine monophosphate synthetase (UMPS), respectively disrupting the purine and pyrimidine de novo synthesis pathways.
Comments: Characteristics: Useful for the production of recombinant monoclonal antibodies as it rovides a rapid and efficient selection method for the serial or simultaneous transfer of genes for multiple polypeptides of choice into the cells using readily available purine- and pyrimidine-free commercial media.
Comments: Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3I1V4; Atic.
Comments: Knockout cell: Method=CRISPR/Cas9; UniProtKB; G3GSP0; Umps.
Comments: Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Comments: Cell type: Epithelial cell of ovary; CL=CL_2000064.
Species of origin:NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
Hierarchy:CVCL_0214 ! CHO-K1
Sex of cell:Female
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_ZE99
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® ID CHO-K1 UA10 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

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