CRISPRi Gen1C 5人诱导性多能干细胞NTCC® Human Induced pluripotent stem cell BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID CRISPRi Gen1C 5 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: WTC CRISPRi Gen1 clone 5; CRISPRi (Gen1C 5); 1C-5
Cross-references: Wikidata; Q93458763
References identifiers: PubMed=26971820;
References identifiers: PubMed=30290179;
Comments: Population: Japanese.
Comments: Characteristics: Cell line with Dox-inducible CRISPR interference (CRISPRi).
Comments: Characteristics: Using TALEN a construct containing a rtTA+TRE3G promoter and dCas9-KRAB-P2A-mCherry is introduced in the AAVS1 safe harbor locus.
Comments: Genetic integration: Method=TALEN; Gene=FPbase; ZERB6; mCherry (Note=Red fluorescent protein derived from drFP583 (dsRed)).
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; P06492; Human herpesvirus 1 VP16 (UL28) (Note=With p.Met1_Arg360del).
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Comments: Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_VM38
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID CRISPRi Gen1C 5 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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