CRISPRi Gen3C A3人诱导性多能干细胞NTCC® Human Induced pluripotent stem cell BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID CRISPRi Gen3C A3 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: WTC CRISPRi Gen3 clone A3; 3C-A3
Cross-references: Wikidata; Q93458772
References identifiers: PubMed=26971820;
Comments: Population: Japanese.
Comments: Characteristics: Cell line with constitutive CRISPR interference (CRISPRi).
Comments: Characteristics: Using TALEN a construct containing a CAG promoter and dCas9-KRAB is introduced in the AAVS1 safe harbor locus.
Comments: Genetic integration: Method=TALEN; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9 (Note=With p.Asp10Ala and p.His840Ala and KRAB domain = dCas9-KRAB).
Comments: Derived from site: In situ; Leg, skin; UBERON=UBERON_0001511.
Comments: Cell type: Fibroblast of skin; CL=CL_0002620.
Species of origin:NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy:CVCL_Y803 ! GM25256
Sex of cell:Male
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC®- CVCL_VM42
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[Reference参考文献]
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[Date保藏时间]
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培养方法：
Medium培养基：NTCC® ID CRISPRi Gen3C A3 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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