HEK293 HL10人转化细胞株NTCC® Human Transformed cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID HEK293 HL10 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HL10; Human PRLR-Luciferase clone 10
References identifiers:PubMed=12668884;
Comments:Characteristics: Used in a transcriptional bioassay for lactogens. Upon binding a lactogen to PRLR a complex is generated that activates JAK2 which in turn activates STAT5 which results in transcriptional activation of the luciferase reporter gene. Thus, the luciferase activity reflects receptor activation by the exogenous PRLR ligand (PubMed=12668884).
Comments:Characteristics: Transfected with two constructs, one containing PRLR under the control of a CMV promoter and the other a firefly luciferase reporter gene under the control of the HSV-TK promoter containing a six-repeat lactogenic hormone response element (LHRE) (PubMed=12668884).
Comments:Genetic integration: Method=Transfection; Gene=HGNC; HGNC:9446; PRLR.
Comments:Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Firefly luciferase.
Comments:Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments:Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Comments:Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin:NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy:CVCL_0045 ! HEK293
Sex of cell:Female
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[Disease疾病种类]
[编号]Accession: NTCC® CVCL_E7I7
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培养方法：
Medium培养基：NTCC® ID HEK293 HL10 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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