HEK293-DR-GFP-XRCC2-13/X2+人转化细胞株NTCC® Human Transformed cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID HEK293-DR-GFP-XRCC2-13/X2+ Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Cross-references:DSMZ; ACC-853
Cross-references:DSMZCellDive; ACC-853
Cross-references:Wikidata; Q94095077
Comments:Characteristics: The parental XRCC2 KO cell line is functionally reconstituted by retroviral transfection of XRCC2.
Comments:Characteristics: Transfected with the hprtDR-GFP reporter construct that targets the DR-GFP into the hprt locus and includes a dominant selectable puroR marker. The DR-GFP construct which contains a tandem repeat of two inactive genes coding for EGFP. After transfection with an expression vector coding for the I-SceI enzyme, recombination induced by the cleavage of the I-SceI site recreates a functional EGFP.
Comments:Characteristics: Transfected with the tetracycline transactivator (tTA) protein, a fusion of tetR with the activation domain of HSV-1 VP16.
Comments:Doubling time: ~40 hours (DSMZ=ACC-853).
Comments:Knockout cell: Method=CRISPR/Cas9; HGNC; HGNC:12829; XRCC2.
Comments:Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments:Genetic integration: Method=Transfection/transduction; Gene=HGNC; HGNC:12829; XRCC2.
Comments:Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).
Comments:Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P04483; Escherichia coli Tn10 tetR.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC® CVCL_XX09
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID HEK293-DR-GFP-XRCC2-13/X2+ Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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