HEK293-SREBP1-T2A-luciferase-KI人转化细胞株NTCC® Human Transformed cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID HEK293-SREBP1-T2A-luciferase-KI Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Cross-references:Wikidata; Q94095267
References identifiers:PubMed=30232659;
Comments:Characteristics: Useful for studying transcriptional regulation of the SREBP1 gene and for screening drugs or chemical molecules that regulate SREBP1 gene expression (PubMed=30232659).
Comments:Characteristics: CRISPR/Cas9-mediated knock-in of a T2A-luciferase-CMV-eGFP-T2A-Neomycin construct at the C-terminal end of SREBP1.
Comments:Genetic integration: Method=Transfection/transduction; Gene=FPbase; R9NL8; eGFP (Note=Enhanced GFP).
Comments:Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P08659; Firefly luciferase.
Comments:Genetic integration: Method=Transfection/transduction; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments:Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Comments:Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
Species of origin:NCBI_TaxID=9606; ! Homo sapiens (Human)
Hierarchy:CVCL_0045 ! HEK293
Sex of cell:Female
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC® CVCL_VR42
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® ID HEK293-SREBP1-T2A-luciferase-KI Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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