HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3人转化细胞株NTCC® Human Transformed cell line BioVector NTCC细胞保藏中心

[Designation名称] NTCC® ID HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HEK alpha-synuclein (SNCA) FRET reporter
Cross-references:Wikidata; Q127381679
Comments:Characteristics: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters (Direct_author_submission).
Comments:Genetic integration: Method=Transfection; Gene=FPbase; RYFE4; mClover3 (Note=Basic green/yellow fluorescent protein derived from GFP).
Comments:Genetic integration: Method=Transfection; Gene=FPbase; MZMFB; mRuby3 (Note=Bright red fluorescent protein derived from eqFP611).
Comments:Genetic integration: Method=Transfection; Gene=HGNC; HGNC:11138; SNCA (Note=With p.Ala53Thr).
Comments:Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Comments:Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Comments:Transformant: NCBI_TaxID; 28285; Adenovirus 5.
Comments:Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Comments:Miscellaneous: Cell line information from personal communication of Yuste-Checa, Patricia.
Comments:Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
[Organism物种来源]
[Disease疾病种类]
[编号]Accession: NTCC® CVCL_D4BV
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[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® ID HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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