32Dcl3小鼠造血祖细胞珠mouse hematopoietic progenitor cell line BioVector NTCC典型培养物保藏中心

NTCC's 32Dcl3 cell line is a widely used model in cellular and molecular biology, particularly in the study of hematopoiesis, cell proliferation, differentiation, and apoptosis.

Origin and Characteristics

• Origin: The 32Dcl3 cell line is a murine (mouse) hematopoietic progenitor cell line, derived from the bone marrow of a C3H/HeJ mouse.

• Cell Type: It is an immature myeloid cell line, meaning it is a precursor to a type of white blood cell.

• Growth Factor Dependence: A defining characteristic of 32Dcl3 cells is their strict dependence on Interleukin-3 (IL-3) for survival and proliferation.

• Differentiation: In the presence of Granulocyte-Colony Stimulating Factor (G-CSF) and in the absence of IL-3, these cells can be induced to terminally differentiate into morphologically and functionally mature neutrophils. This makes them a valuable tool for studying the process of granulocytic differentiation.

• Nontumorigenic: The cell line is considered nontumorigenic, which is a key advantage for studying normal hematopoietic processes compared to cell lines of leukemic origin.

• Chromosomal Instability: It's important to note that the cell line is known to be chromosomally unstable, with different clones potentially having different karyotypes. This can lead to variations between experiments and laboratories.

Research Applications

The 32Dcl3 cell line is used in various research areas, including:

• Hematopoiesis: It serves as a model for studying the intricate processes of blood cell development and the molecular mechanisms that control cell proliferation and differentiation.

• Signal Transduction: Researchers use these cells to investigate the signaling pathways activated by growth factors like G-CSF and IL-3, and how these signals lead to either proliferation or differentiation.

• Apoptosis: The cells are a well-established model for studying apoptosis (programmed cell death). When IL-3 is withdrawn from the culture medium, the cells undergo apoptosis, allowing for the study of the signaling pathways involved in cell survival and death.

• Neutrophil Function: Differentiated 32Dcl3 cells, which closely resemble normal neutrophils, are used to study the functions of mature neutrophils, such as phagocytosis, degranulation, and adhesion.

• Oncogenesis: The cell line has been used to study how oncogenes, such as v-abl and v-Ha-ras, can disrupt normal differentiation and lead to neoplastic transformation.

Culture Conditions

• Medium: RPMI-1640 medium supplemented with fetal bovine serum (FBS) and a source of mouse IL-3 is typically used.

• IL-3 Source: IL-3 can be supplied as recombinant IL-3 or through a conditioned medium from a cell line that secretes IL-3, such as WEHI-3B.

• Temperature and CO2: Cultures are maintained at 37°C in a 5% CO2 atmosphere.

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