NTCC™ 804G.003A 大鼠尿路上皮细胞株transformed rat urothelial cell line BioVector典型培养物保藏中心

NTCC™ 804G.003A 大鼠尿路上皮细胞系

804G.003A 细胞系是一种转化过的大鼠尿路上皮细胞系，来源于患有膀胱鳞状细胞癌的雄性 ACI 大鼠。它是其亲本细胞系 804 G 的一个亚克隆，是细胞生物学研究中广泛使用的模型，特别是在研究上皮细胞粘附和特殊连接结构形成方面。

主要特点

• 来源: 该细胞系是利用致癌物 N-丁基-N-(3-羧丙基)亚硝胺 (BCPN) 诱导产生的大鼠膀胱肿瘤中建立的。

• 细胞类型: 它是一种尿路上皮细胞系，起源于尿路内壁的上皮组织。

• 粘附特性: 804G.003A 细胞系的一个决定性特征是其形成半桥粒的能力。半桥粒是复杂的细胞结构，用于将上皮细胞锚定在其下方的基底膜上。这使其成为研究这些细胞粘附复合物的组装、调节和功能的经典模型。

• 永生化: 这些细胞是经过转化的，这意味着它们的寿命得以延长，可以无限期地进行培养，这与原代细胞不同。

研究应用

804G.003A 细胞系是细胞和分子生物学研究人员的重要工具。其主要应用包括：

• 研究细胞粘附: 它是研究半桥粒形成分子机制以及其中涉及的整合素和斑珠蛋白等蛋白质的经典模型。

• 上皮生物学: 研究人员用它来了解尿路上皮的结构和功能，尿路上皮是构成尿路内壁的特殊上皮组织。

• 癌症研究: 作为一种肿瘤来源的细胞系，它可用于研究膀胱癌发展过程中细胞粘附和生长所发生的变化。

The 804G.003A cell line is a transformed rat urothelial cell line derived from a male ACI rat with a urinary bladder squamous cell carcinoma. It is a subclone of the parent 804 G cell line and is a widely used model in cell biology research, especially for studying epithelial cell adhesion and the formation of specialized junctions.

Key Characteristics

• Origin: The cell line was established from a rat urinary bladder tumor induced by a carcinogen, specifically N-butyl-N-(3-carboxypropyl)nitrosamine (BCPN).

• Cell Type: It is a urothelial cell line, which means it originates from the lining of the urinary bladder.

• Adhesion Properties: A defining characteristic of the 804G.003A cell line is its ability to form hemidesmosomes, which are complex structures that anchor epithelial cells to the underlying basement membrane. This makes it a primary model for studying the assembly, regulation, and function of these cell adhesion complexes.

• Immortalization: The cells are transformed, meaning they have a prolonged lifespan and can be cultured indefinitely, unlike primary cells.

Research Applications

The 804G.003A cell line is a fundamental tool for researchers in cell and molecular biology. Its main applications include:

• Studying Cell Adhesion: It is a classic model for investigating the molecular mechanisms of hemidesmosome formation and the proteins involved, such as integrins and plectin.

• Epithelial Biology: Researchers use it to understand the structure and function of the urothelium, the specific type of epithelial tissue that lines the urinary tract.

• Cancer Research: As a tumor-derived cell line, it can be used to study the changes in cell adhesion and growth that occur during the development of bladder cancer.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net