pRM1载体 BioVector NTCC中国质粒载体菌种细胞基因保藏中心

pRM1载体

Name  pRM1

Type Plasmid

Class Recombinant

Literature Pla et al., Gene 165 (1995), 115-120 [PMID: 7489899 ]

Related_literatureCannon et al., Mol. Gen. Genet. 235 (1992), 453-457 [PMID: 1465116 ]Cannon et al., Mol. Gen. Genet. 221 (1990), 210-218 [PMID: 2196431 ]Kelly et al., Mol. Gen. Genet. 214 (1988), 24-31 [PMID: 2976118 ]Herreros et al., Mol. Microbiol. 6 (1992), 3567-3574 [PMID: 1474898 ]

Host_for_distribution Escherichia coli K12 MC1061

Selectable_phenotype Ampicillin (amp)

Candida albicans URA3; auxotrophic

Candida albicans LEU2; auxotrophic

Replicon Escherichia coli plasmid pMB1 origin Candida albicans autonomously replicating sequence ARS2 Candida albicans autonomously replicating sequence ARS3Host_rangeEscherichia coliCandida albicans; leu2(-), ura3(-)PromoterEscherichia coli lac operon promoter

Further_information

The construction of this plasmid is described by Pla et al. (1995).pRM1 is a non-integrative, double-ARS shuttle vector for Candida albicans strains.The presence of both C. albicans autonomously replicating sequences ARS2 and ARS3 allows direct recovery in Escherichia coli of non-multimerized plasmids from C. albicans transformants without any intermediate step in Saccharomyces cerevisiae.When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.The nucleotide sequence of the Candida URA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198 (version SV 1).

>ENA|X14198|X14198.1 Candida albicans URA3 gene for orotidine-5'-monophosphate decarboxylase sequenceAGTACTAATAGGAATTGATTTGGATGGTATAAACGGAAACAAAAAAAAGAGCTGGTACTACTTTCTTTAAAATTATTTTATTATTTGATTTTATTTAATAGTATATATTATATTTTGAACGTAGATTATTTTGTTGAAAGTTGCTGTAGTGCCATTGATTCGTAACACTAATTCTGTATTAGTCATTCCTCTTGTTTGATAGTATCCAAAAAAACGGCTATTTTTTTGCAATCTTATTTCCTGCATATTATACAGATAACATAATGAAAGAAAAAATCTTTTTTTTTGTTCTTCAATGATGATTTCAACCATTCTTTTAAACATTGATCAATTCCTGAGCAACAACCCCATACACACTGGTTTATATACCGCCCCTTTTACAGTTGAAGAAAGAAATAGAAATAGAAATAGCAAACAAAAGATATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAATTCCTTGAATTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAAATGTGAAGGGGGAGATTTTCACTTTATTAGATTTGTATATATGTAGAATAAATAAATAAATAAGTTAAATAAATAATTAAATAAGGGTGGTAATTATTACTATTTACAATCAAAGGTGGTCCTTCTAGA

BioVector NTCC中国质粒载体菌种细胞基因保藏中心