Multiplex CRISPR/Cas9 Assembly System Kit, 1000000055 BioVector NTCC中国质粒载体菌种细胞基因保藏中心
- 价 格：¥19300
- 货 号：Multiplex CRISPR/Cas9 Assembly System Kit, 1000000055
- 产 地：北京
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Multiplex CRISPR/Cas9 Assembly System Kit, 1000000055
This 18 plasmid CRISPR kit is used to construct all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase. A separate accessory packis available for dCas9 and FokI-dCas9.
This kit consists of one 96-well plate, and will be shipped as bacterial glycerol stocks on dry ice. Samples should be frozen at -80°C immediately upon arrival. Individual plasmids can be ordered at $65 per plasmid, and will be shipped as bacterial stabs
Original Publication
Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system. Sakuma T, Nishikawa A, Kume S, Chayama K, Yamamoto T. Sci Rep. 2014 Jun 23;4:5400. doi: 10.1038/srep05400. PubMed .
Description
The Multiplex CRISPR/Cas9 Assembly System Kit enables construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs (2-7) and a Cas9 nuclease or nickase . Multiple gRNA cassettes are assembled using BsaI-mediated Golden Gate cloning method. The kit contains 18 plasmids: pX330A-1x[2-7], pX330A_D10A-1x[2-7], and pX330S-[2-7].
Overview of Multiplex CRISPR/Cas9 assembly
Figure from Sakuma et al., Sci Rep . 2014 Jun 23;4:5400.
BioVector NTCC中国质粒载体菌种细胞基因保藏中心
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