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FOPFlash,TOPFlash 载体质粒图谱序列抗性价格报价Biovector NTCC Inc.现货

  • 价  格:¥4920
  • 货  号:BiovectorFOPFlash,TOPFlash
  • 产  地:北京
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FOPFlash 载体

基本信息

质粒类型:信号通路报告载体
克隆方法:多克隆位点,限制性内切酶


Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPFLASH (Catalog # 21-170) which contains wild type Tcf binding sites.

Background

Transfection grade T cell factor (Tcf) reporter plasmid containing three copies of the Tcf binding site (mutated) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPFLASH (Catalog # 21-170) which contains wild type Tcf binding sites.



TOPFlash 载体

出品公司:Millipore
质粒类型:信号通路报告载体
克隆方法:多克隆位点,限制性内切酶
载体抗性:Ampicillin (氨苄青霉素)


The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. Dishevelled down regulates glycogen synthase kinase-3 (GSK-3 ) activity. This results in the stabilization and accumulation of cytosolic -catenin, a GSK-3 substrate, which when phosphorylated by GSK-3, is targeted for ubiquitin-mediated proteolysis. -catenin then translocates to the nucleus, where in complexes with members of the Tcf/LEF family of transcription regulators, activates transcription of Tcf-responsive genes. This Tcf-reporter plasmid, along with FOPFLASH (Cat. # 21-169) enables quantitation of Wnt/Wg signaling in cells transfected with these constructs.


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