HEK293T clone 18细胞-BioVector NTCC®细胞保藏中心

[Designation名称] NTCC® Cell Name: Tau RD P301L FRET Sensitive Biosensor Cell Line细胞株
BioVector NTCC典型培养物保藏中心

Synonyms: HEK293T clone 18
Cross-references: Wikidata; Q123033603
RX PubMed=37553663;
Comments: Characteristics: Transduced with a lentivirus construct EF1a-TauRD(P301L)-RLinker-mTurquoise2-T2A-TauRD(P301L)-RLinker-mNeonGreen-WPRE where TauRD(P301L) is the tau repeat domain (RD) with the disease-associated p.Pro301Leu (p.Pro636Leu) mutation and Rlinker is a (EAAAK)3 rigid linker (PubMed=37553663).
Comments: Characteristics: Shows a high sensitivity in detecting bioactive tau seeds. These seeds can nucleate the aggregation of the reporter proteins. This aggregation produces a FRET signal (PubMed=37553663).
Comments: Genetic integration: Method=Transduction; Gene=FPbase; ZRKRV; mNeonGreen (Note=Derivative of Branchiostoma lanceolatum LanYFP).
Comments: Genetic integration: Method=Transduction; Gene=FPbase; 7AV5G; mTurquoise2 (Note=Tq2, cyan fluorescent protein derived from GFP).
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Comments: Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Comments: Transformant: NCBI_TaxID; 28285; Human adenovirus C serotype 5.
Comments: Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA).
Comments: Derived from site: In situ; Fetal kidney; UBERON=UBERON_0002113.
[Organism物种来源]
[Disease疾病种类]
[编号]NTCC® Accession: CVCL_C8YL
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[Reference参考文献]
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[Date保藏时间]
[Synonyms别名]
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培养方法：
Medium培养基：NTCC® Cell Name: Tau RD P301L FRET Sensitive Biosensor Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.
Atmosphere: 95% Air, 5% CO2

Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
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