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[Designation名称] NTCC® Cell Name: U87-MG/Cas9 AAVS1 Cell Line细胞株
BioVector NTCC典型培养物保藏中心Synonyms: U87-MG/Cas9-AAVS1Cross-references: GeneCopoeia; SL584Cross-references: Wikidata; Q123033672Comments: Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.Comments: Population: Caucasian.Comments: Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL584).Comments: Sequence variation: Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
[Organism物种来源]
[Disease疾病种类]
[编号]NTCC® Accession: CVCL_C9F7
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Cell Name: U87-MG/Cas9 AAVS1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Ordering下单订购网站 http://www.biovector.net/product/286676.html
BioVector NTCC典型培养物保藏中心Synonyms: U87-MG/Cas9-AAVS1Cross-references: GeneCopoeia; SL584Cross-references: Wikidata; Q123033672Comments: Problematic cell line: Misidentified. Parent cell line (U-87MG ATCC) is not the original glioblastoma cell line established in 1968 at the University of Uppsala.Comments: Population: Caucasian.Comments: Characteristics: Stably expresses a CBh-3xFLAG-Cas9-NLS-bGHPolyA-EF1a-copGFP-T2A-HygR-SV40pa construct from the AAVS1 safe locus (GeneCopoeia=SL584).Comments: Sequence variation: Mutation; HGNC; HGNC:7765; NF1; Simple; p.Phe1247Ilefs*18 (c.3739_3742delTTTG) (3737_3740delTGTT); ClinVar=VCV000420078; Zygosity=Heterozygous (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:9588; PTEN; Simple; c.209+1G>T; ClinVar=VCV000229705; Zygosity=Homozygous; Note=Splice donor mutation (from parent cell line).Comments: Sequence variation: Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).Comments: Genetic integration: Method=Transduction; Gene=FPbase; 2BK6M; ppluGFP2 (copGFP, green fluorescent protein from P.plumata).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; P00557; Escherichia coli hygromycin-B 4-O-kinase (hph) (HygR).Comments: Genetic integration: Method=Transduction; Gene=UniProtKB; Q99ZW2; Streptomyces pyogenes Cas9.
[Organism物种来源]
[Disease疾病种类]
[编号]NTCC® Accession: CVCL_C9F7
[Comments描述]
[Reference参考文献]
[Category分类]
[Date保藏时间]
[Synonyms别名]
[Hierarchy]
培养方法：
Medium培养基：NTCC® Cell Name: U87-MG/Cas9 AAVS1 Complete Medium
Growth properties形态：贴壁/悬浮：
Culture Condition培养条件：Temperature温度: 37°C.Atmosphere: 95% Air, 5% CO2
Supplier生产厂家: BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
Ordering下单订购网站 http://www.biovector.net/product/286676.html
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