BT-C牛滋养层细胞株NTCC® bovine trophoblast cell line

The NTCC® BT-C cell line is one of several established bovine trophoblast cell lines (BT-A to BT-L) used as an in vitro model for studying the development and function of the bovine placenta and the maternal-fetal interface.

Key Characteristics and Use

• Origin: The cell lines (BT-A to BT-L) were established from in vitro fertilized bovine embryos.

• Purpose: BT-C and related cell lines are valuable tools for researchers because they can differentiate into mature trophoblast cells, including the formation of characteristic binucleate giant cells, under specific culture conditions.

• Differentiation: Trophoblast cells play a pivotal role in the establishment of pregnancy and placental development in cattle. These cell lines express key markers such as cytokeratin, and can be induced to express pregnancy-associated proteins like placental lactogen (PL1), prolactin-related protein-1 (PRP1), and pregnancy-associated glycoprotein-1 (PAG1).

• Research Applications: They are used in studies involving trophoblast differentiation, binucleation (the process of forming binucleate cells which then fuse with uterine epithelial cells), and the regulation of cell invasiveness.

• Specific Properties: In one study examining the expression of the gene SOLD1, the BT-C cell line was noted to have one of the lowest expression levels of the gene among the lines examined, and its invasiveness was not affected by anti-bovine SOLD1 antibodies, unlike the BT-K line. This highlights the variable characteristics among different BT cell lines.

• Handling: BT-C cells are considered somewhat difficult to handle compared to typical cancer or fibroblast cell lines.

• Growth: They grow in a colony-like fashion and can form dome-like structures (vesicles).

• Maintenance: Special attention to culture techniques is necessary, such as not fully dissociating the cells during passaging and using an increased concentration of Fetal Bovine Serum (FBS) during the freeze-thaw process

• 起源： BT-C细胞系（JCRB1866）建立自体外受精的牛胚泡（胚囊）滋养层。

• 用途： 它是研究牛滋养层细胞分化、胎盘发育以及早期妊娠失败机制的重要工具。研究人员利用这些细胞系探讨滋养层细胞的侵袭性（invading ability）调控等过程。

• 分化能力： 在特定的培养条件下，BT-C细胞可以分化成成熟的滋养层细胞，包括形成特征性的双核巨细胞（binucleate giant cells）。

• 标记物表达： 这些细胞表达细胞角蛋白（cytokeratin）等关键滋养层标记物，并且可以被诱导表达妊娠相关蛋白，例如胎盘生乳素（PL1）、催乳素相关蛋白-1（PRP1）和妊娠相关糖蛋白-1（PAG1）。

• 科研价值： BT-C细胞系有助于科学家了解牛胚胎着床前后的复杂信号传导和母胎对话过程。

• 培养难度： 相比于常见的癌细胞系或成纤维细胞系，BT-C细胞系通常被认为处理起来“有些难度”。

• 生长方式： 它们倾向于以克隆（集落）方式生长，并能形成圆顶状结构（囊泡）。

• 维护要求： 在传代过程中，需要注意培养技术，例如不应将细胞完全解离成单个细胞，并在冻存/复苏过程中使用更高浓度的胎牛血清（FBS）。

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

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