SODk1 NTCC®源自人胚胎肾细胞慢病毒稳定包装细胞株-用于生产高效价、四环素调控的慢病毒载体

NTCC®

• 起源： 该细胞系源自人胚胎肾293（HEK293）细胞。

• 功能： 它作为逆转录病毒包装细胞系发挥作用，设计用于在诱导后产生慢病毒载体颗粒。

• 诱导系统： 该系统使用Tet-off诱导启动子系统。在抗生素强力霉素（Dox）存在时，基因表达被抑制。撤除Dox后，病毒成分（包装蛋白和VSV-G包膜蛋白）的产生被激活。

• 安全性： 它被开发用于生成高效价的自失活（SIN）载体，与早期的慢病毒系统相比，这些载体具有更高的安全性，因为它们在靶细胞中保留了自失活特性。

• 增强效果： 添加丁酸钠（一种脱乙酰化抑制剂）可通过影响染色质重塑，显著提高基因表达水平和产生的病毒滴度。

• 应用： SODk1细胞产生的载体可以有效转导分裂细胞和非分裂细胞，包括大鼠大脑中终末分化的神经元，这使得它们在体内基因递送研究中非常有价值

• Origin: The cell line is derived from human embryonic kidney 293 (HEK293) cells.

• Function: It functions as a retrovirus packaging cell line, designed to produce lentiviral vector particles upon induction.

• Inducible System: The system uses a Tet-off inducible promoter system. In the presence of the antibiotic doxycycline (Dox), gene expression is suppressed. Upon Dox withdrawal, the production of viral components (packaging proteins and the VSV-G envelope protein) is activated.

• Safety: It was developed to generate high-titer SIN (self-inactivating) vectors, which have enhanced safety features compared to earlier lentiviral systems as they retain their self-inactivating properties in target cells.

• Enhancement: The addition of sodium butyrate, a deacetylation inhibitor, significantly increases the magnitude of gene expression and the resulting vector titers by affecting chromatin remodeling.

• Application: The vectors produced by SODk1 cells can efficiently transduce both dividing and non-dividing cells, including terminally differentiated neurons in rat brains, making them valuable for in vivo gene delivery studies.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心

BioVector NTCC Inc.

TEL: 400-800-2947, 189-0126-8599

E-mail: biovector@163.com

http://www.biovector.net